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In addition for lungs, one lobe was stored in RNA Later (Ambion, ThermoFisher Scientific, Illkirch, France) for transcriptomic analyses, posterior lobes were fixed with paraformaldehyde (PFA 4%, Labonord, Villeneuve dAscq, France) for histopathological analyses, and another lobe was frozen in liquid nitrogen and then stored at 80 C until further analyses

The smoke box test took 59 seconds, nearly 6x longer than the LEVOIT

While a traditional cigarette burns at extreme temperatures of up to 900C, creating toxic smoke, ash, and tar, HNB devices maintain a strictly controlled environment (typically between 250C and 350C)

The other is to the state, which in an effort to squeeze $3.00 from a pack instead loses the $2.70 it was already getting, plus another roughly 77 cents in lost sales tax. Excise taxes like those that New Jersey levies on nicotine products have dual roles